GSM6716999: 61-AO3 E2-ER-Rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ESR1
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
MCF7
cell line
MCF7
cell type
ER+ breast cancer cell line
genotype
sgNT
chip antibody
ER (Millipore, #06-935)
Sequenced DNA Library
library_name
GSM6716999
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching, chromatin was prepared using SimpleChIP Enzymatic Chromatin Kit from cell signaling. ChIP-seq libraries were prepared using the KAPA HTP Library Preparation Kit.